lc-ms法提取微生物提取物microbial extract by lc-ms
dionex 3000rs uhplc system coupled with bruker maxis q-tof ms
ultracore superc18 2.5um, 150 x 2.1mm
gradient analysis
a = 0.1% formic acid in water
b = 0.1% formic acid in acetonitrile
time (mins) %b time (mins) %b
0 5 20 100
5 5 25 100
flow rate: 0.2ml/min
electrospray ms (positive mode)
source: end plate offset: -500v,
capillary: -4500v
nebuliser gas (n2) at 1.6 bar
drying gas (n2) temp.: 180°c
collision energy: 5.0ev
collision rf: 600vpp